Introducing antisense oligonucleotides into Pneumocystis carinii

J Microbiol Methods. 2002 Jul;50(2):211-3. doi: 10.1016/s0167-7012(02)00033-7.

Abstract

To improve the knowledge on Pneumocystis carinii growth, a homologous P. carinii transformation system would provide a tool to promote replication of this fungus. Antisense oligonucleotides have been successfully introduced by electroporation or direct uptake in order to downregulate the prohibitin negative function on cell cycle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Cycle
  • Electroporation / methods*
  • Oligonucleotides, Antisense / genetics
  • Oligonucleotides, Antisense / metabolism*
  • Pneumocystis / growth & development*
  • Pneumocystis / metabolism
  • Pneumonia, Pneumocystis / microbiology
  • Prohibitins
  • Proteins / genetics*
  • Rats
  • Rats, Sprague-Dawley
  • Repressor Proteins*
  • Transformation, Genetic*

Substances

  • Oligonucleotides, Antisense
  • Prohibitins
  • Proteins
  • Repressor Proteins