This paper describes a simple method for the isolation of salt-tolerant myxobacteria from marine conditions. As the results show in this paper, salt-tolerant myxobacteria are found to be able to grow, but unable to form fruiting bodies at high salt concentrations. The fruiting body structures of the salt-tolerant strains were all formed in conditions with lower seawater content, i.e. lower than 60% seawater (about 2.0% salt content) or distilled water supplemented with MgCl(2). The method picked up the fruiting bodies for isolation.