Synthesis and immunofluorescence assay of a new biotinylated paclitaxel

Eur J Med Chem. 2002 Apr;37(4):349-53. doi: 10.1016/s0223-5234(02)01337-5.

Abstract

7-(5'-Biotinylamidopropanoyl)paclitaxel was synthesised by chemical methods; its immunofluorescence assay and the cell uptake experiments were performed by use of human leukemia U937 cells. The results indicate that paclitaxel is arresting cell cycle at the G(2)M phase only.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / chemical synthesis*
  • Antineoplastic Agents / metabolism*
  • Fluorescent Antibody Technique
  • Fluorescent Dyes / chemistry
  • Humans
  • Microtubules / metabolism
  • Paclitaxel / analogs & derivatives
  • Paclitaxel / chemical synthesis*
  • Paclitaxel / metabolism*
  • Taxoids*
  • Tumor Cells, Cultured
  • U937 Cells

Substances

  • 7-(5'-Biotinylamidopropanoyl)paclitaxel
  • Antineoplastic Agents
  • Fluorescent Dyes
  • Taxoids
  • Paclitaxel