Production and analysis of neurospheres from acutely dissociated and postmortem CNS specimens

Methods Mol Biol. 2002:198:15-27. doi: 10.1385/1-59259-186-8:015.
No abstract available

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Animals, Newborn
  • Brain Tissue Transplantation / instrumentation
  • Brain Tissue Transplantation / methods*
  • Cell Aggregation / drug effects
  • Cell Aggregation / physiology
  • Cell Culture Techniques / instrumentation
  • Cell Culture Techniques / methods*
  • Cell Differentiation / drug effects
  • Cell Differentiation / physiology
  • Cell Division / drug effects
  • Cell Division / physiology
  • Cells, Cultured / drug effects
  • Cells, Cultured / metabolism
  • Cells, Cultured / ultrastructure*
  • Central Nervous System / growth & development
  • Central Nervous System / metabolism
  • Central Nervous System / ultrastructure
  • Cloning, Molecular / methods
  • Culture Media
  • Genetic Therapy / instrumentation
  • Genetic Therapy / methods*
  • Growth Substances
  • Humans
  • Immunohistochemistry / instrumentation
  • Immunohistochemistry / methods
  • Mice
  • Microscopy, Electron
  • Neuroglia / drug effects
  • Neuroglia / metabolism
  • Neuroglia / ultrastructure
  • Neurons / drug effects
  • Neurons / metabolism
  • Neurons / ultrastructure*
  • Postmortem Changes
  • Reverse Transcriptase Polymerase Chain Reaction
  • Spheroids, Cellular / drug effects
  • Spheroids, Cellular / metabolism
  • Spheroids, Cellular / ultrastructure*
  • Stem Cells / drug effects
  • Stem Cells / metabolism
  • Stem Cells / ultrastructure*

Substances

  • Culture Media
  • Growth Substances