2,6-diaminopurine in TNA: effect on duplex stabilities and on the efficiency of template-controlled ligations

Xiaolin Wu; Guillermo Delgado; Ramanarayanan Krishnamurthy; Albert Eschenmoser

doi:10.1021/ol020016p

2,6-diaminopurine in TNA: effect on duplex stabilities and on the efficiency of template-controlled ligations

Org Lett. 2002 Apr 18;4(8):1283-6. doi: 10.1021/ol020016p.

Authors

Xiaolin Wu¹, Guillermo Delgado, Ramanarayanan Krishnamurthy, Albert Eschenmoser

Affiliation

¹ The Skaggs Institute for Chemical Biology at the Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.

PMID: 11950343
DOI: 10.1021/ol020016p

Abstract

Replacement of adenine by 2,6-diaminopurine-two nucleobases to be considered equivalent from an etiological point of view-strongly enhances the stability of TNA/TNA, TNA/RNA, or TNA/DNA duplexes and efficiently accelerates template-directed ligation of TNA ligands. [reaction: see text]

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

2-Aminopurine / analogs & derivatives*
2-Aminopurine / chemistry*
Base Pairing
Chromatography, High Pressure Liquid
Crystallography, X-Ray
DNA / chemistry
Furans / chemistry*
Ligands
Mass Spectrometry
Oligonucleotides / chemistry*
RNA / chemistry
Spectrophotometry, Ultraviolet
Templates, Genetic

Substances

Furans
Ligands
Oligonucleotides
2-Aminopurine
2,6-diaminopurine
RNA
DNA