Characterization of plasmid pRT1 from Pyrococcus sp. strain JT1

J Bacteriol. 2002 May;184(9):2561-6. doi: 10.1128/JB.184.9.2561-2566.2002.

Abstract

We discovered a 3,373-bp plasmid (pRT1) in the hyperthermophilic archaeon Pyrococcus sp. strain JT1. Two major open reading frames were identified, and analysis of the sequence revealed some resemblance to motifs typically found in plasmids that replicate via a rolling-circle mechanism. The presence of single-stranded DNA replication intermediates of pRT1 was detected, confirming this mode of replication.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Cloning, Molecular
  • DNA Replication
  • DNA, Single-Stranded / analysis
  • Molecular Sequence Data
  • Open Reading Frames
  • Plasmids / chemistry
  • Plasmids / genetics*
  • Pyrococcus / genetics*
  • Sequence Alignment

Substances

  • DNA, Single-Stranded

Associated data

  • GENBANK/AF393813
  • GENBANK/AF411292