[Construction of the immunoenzyme test system "IFA-AtEBV-Strip" for detecting antibodies of the Epstein-Barr virus]

S D Zagorodna; N V Nesterova; N S Diachenko; G V Baranova; S L Rybalko; N V Ivans'ka; L G Vasylenko

[Construction of the immunoenzyme test system "IFA-AtEBV-Strip" for detecting antibodies of the Epstein-Barr virus]

Mikrobiol Z. 2001 Nov-Dec;63(6):61-70.

[Article in Ukrainian]

Authors

S D Zagorodna¹, N V Nesterova, N S Diachenko, G V Baranova, S L Rybalko, N V Ivans'ka, L G Vasylenko

Affiliation

¹ Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154 Zabolotny St., Kyiv, 03143, Ukraine.

PMID: 11944337

Abstract

The first home immunoenzyme test-system for finding antibodies to the Epstein-Barr virus (EBV) has been created. The test-system laboratory prototype was called "IFA-AtEBV-strip". Production technology has been developed for specific components of immunoenzyme test system and all the analysis stage conditions have been optimized: the antigen sorption on plates, its interaction with the studied serum, finding out of the formed immune complex. The test system "IFA-AtEBV-strip" sensitivity made up 96%, specificity--100%.

Publication types

English Abstract

MeSH terms

Antibodies, Viral / blood*
Antigens, Viral / immunology
Herpesvirus 4, Human / immunology*
Humans
Immunoenzyme Techniques / methods*
Reagent Kits, Diagnostic
Sensitivity and Specificity

Substances

Antibodies, Viral
Antigens, Viral
Reagent Kits, Diagnostic