Abstract
A specific inhibitor of DNA cytosine C5-methylases would be useful for studying genomic imprinting, X-chromosome inactivation, carcinogenesis, and regulation of tissue-specific gene expression, for these physiological phenomena appears to be regulated through DNA methylation in promoter sequences. This paper reports a novel convenient in vitro assay method for screening DNA cytosine C5-methylase inhibitor. Our method uses a commercially available Hae III methylase (cytosine C5 methylase), its corresponding Hae III endonuclease, and lambda DNA as their substrate.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine / analogs & derivatives*
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Adenosine / metabolism
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Bacteria
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DNA (Cytosine-5-)-Methyltransferases / antagonists & inhibitors*
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DNA / genetics*
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DNA Restriction-Modification Enzymes
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DNA-Cytosine Methylases / metabolism
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Deoxyribonucleases, Type II Site-Specific / metabolism
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Electrophoresis, Agar Gel / methods
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Enzyme Inhibitors / analysis*
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Escherichia coli / enzymology
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In Vitro Techniques
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S-Adenosylmethionine / metabolism
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Sequence Homology, Nucleic Acid
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X Chromosome / metabolism
Substances
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DNA Restriction-Modification Enzymes
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Enzyme Inhibitors
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S-Adenosylmethionine
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DNA
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DNA-Cytosine Methylases
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Cytosine 5-methyltransferase
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DNA (Cytosine-5-)-Methyltransferases
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Deoxyribonucleases, Type II Site-Specific
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GGCC-specific type II deoxyribonucleases
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Adenosine
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sinefungin