The molecular characterization of maize B chromosome specific AFLPs

Cell Res. 2002 Mar;12(1):63-8. doi: 10.1038/sj.cr.7290111.

Abstract

The origin and evolution of B chromosomes could be explained by the specific DNA sequence on them. But the specific sequences known were quite limited. To investigate maize B chromosome sqicific DNA sequeces, maize genomes with and without B chromosomes were analyzed by AFLP. Only 5 markers were found specific to genomes with B chromosomes among about 2000 AFLP markers. Southern hybridization and sequence analysis revealed that only the sequence of M8-2D was a B chromosome specific sequence. This sequence contained the telomeric repeat unit AGG

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Centromere / chemistry
  • Chromosomes, Plant / chemistry*
  • Conserved Sequence
  • DNA, Plant / genetics*
  • Genetic Markers
  • In Situ Hybridization, Fluorescence
  • Phylogeny
  • Polymorphism, Restriction Fragment Length*
  • Sequence Analysis, DNA
  • Tandem Repeat Sequences
  • Telomere / chemistry
  • Zea mays / genetics*

Substances

  • DNA, Plant
  • Genetic Markers