Objective: To establish a new nucleic acid hybridization detection technique which may be used in medical genechips.
Methods: The specific DNA fragment was detected by sequential two hybridization of fluorescence probe with template DNA and fixed DNA probe.
Results: Fluorescence probe two-hybridization (FPTH) was applied to genechips for the detection of sex-transmitted pathogens from culture strains, and the results showed that the values of fluorescence density of the positive groups decreased remarkably when compared with those of the negative group. Both the sensitivity and specificity for detecting clinical samples are higher than 90%. There is no need of any additional reagent in hybridization procedure, and the hybridization detection can be accomplished in 40 minutes.
Conclusion: The FPTH technique is rapid, simple and reliable, it can also make the clinical detection process completely automatic and integrative.