Objective: To prepare transgenic mice model of human Swedish mutation of amyloid precursor protein(APP(SWE)) gene.
Methods: By pronuclear microinjection, human APP (SWE) genes were microinjected into pronuclei of zygotes of C57 and Kunming white mice. Integral zygotes were implanted into the oviducts of pseudopregnant female mice. The integration and expression of exogeneous genes in offsprings were detected by polymerase chain reaction (PCR), fluorescence in situ hybridization (FISH) and reverse transcription-ploymerase chain reaction (RT-PCR) analysis.
Results: The success rate of microinjection and the birth rate were 76.62% and 10.38%, respectively. There were 6 founders and the integration rate was 35.29%. The exogeneous gene was transferred to the third generation. Expression of human APP(SWE) gene was detected in the brain, heart and muscle but not detected in the liver and kidney of transgenic mice.
Conclusion: The transgenic mice model of human APP(SWE) gene has been prepared successfully.