Ultrasound investigation of aortic blood flow (ABF) still represents a technically challenging task, because of the complex geometry of such a deep artery. In this paper, we present a unique experimental set-up capable of providing detailed information about blood dynamics in the aorta. The set-up is based on an esophageal probe (EP) connected to a multigate Doppler-processing system. The EP, developed for the noninvasive hemodynamic monitoring of ABF in patients under general anesthesia or in the intensive care area, must be inserted at a thoracic depth where the esophagus and the aorta are nearby and parallel. Doppler processing of pulsed wave echoes in the multigate system provides the distribution of all Doppler frequencies detected along the probe beam axis (spectral profile) in real time. The results of this investigation confirm that flow in the aorta is extremely complex, especially at the level of the aortic arch or in nonphysiologic circumstances. In general, the velocity profiles tend to be flat only during the systolic acceleration, but not during the full cardiac cycle. In most cases, they are asymmetrical, including both positive and negative components. In particular, it is shown that an appropriate positioning of the ultrasound transducer and/or the correct integration of different velocities is mandatory to make reliable ABF measurements.