Abstract
Immunohistochemistry for calcitonin gene-related peptide (CGRP) was performed on the dorsal root ganglion (DRG) and spinal cord in wildtype and knockout mice for Brn-3a. CGRP-immunoreactive (-IR) neurons were abundant in the DRG of wildtype, heterozygous and knockout mice. Cell size analysis revealed that CGRP-IR neurons were of various sizes in wildtype and heterozygous mice. In the knockout mice, however, most of CGRP-IR neurons were small. In the spinal cord of knockout mice, the number of CGRP-IR fibers increased in the dorsal column but decreased in the deep part of the dorsal horn. The loss of Brn-3a may have different effects on CGRP-IR expression in small and large DRG neurons.
MeSH terms
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Animals
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Calcitonin Gene-Related Peptide / analysis*
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Calcitonin Gene-Related Peptide / biosynthesis
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Calcitonin Gene-Related Peptide / genetics
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DNA-Binding Proteins / deficiency*
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DNA-Binding Proteins / genetics*
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Ganglia, Spinal / chemistry*
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Ganglia, Spinal / cytology
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Ganglia, Spinal / metabolism
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Immunohistochemistry / methods
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Mice
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Mice, Knockout
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Mice, Mutant Strains
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Spinal Cord / chemistry
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Spinal Cord / cytology
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Spinal Cord / metabolism
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Transcription Factor Brn-3
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Transcription Factor Brn-3A
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Transcription Factors / deficiency*
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Transcription Factors / genetics*
Substances
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DNA-Binding Proteins
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Pou4f1 protein, mouse
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Transcription Factor Brn-3
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Transcription Factor Brn-3A
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Transcription Factors
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Calcitonin Gene-Related Peptide