The importance of the aflatoxins from food safety and economic standpoints has continued to drive the development of new analytical methods for these mycotoxins. Currently the widely used methods for measurement of aflatoxins fall into two groups, the established chromatographic methods and traditional enzyme-linked immunosorbent assays (ELISAs). Recently substantial progress has been made in the application of new technologies to the monitoring of aflatoxins. In particular, several research groups have developed biosensors for detection ofthe toxins as well as presumptive tests for fungal infection. Biosensors have been developed in a variety of formats including surface plasmon resonance, fiber optic probes, and microbead-based assays. The sensitivity and selectivity of the biosensors and of the presumptive tests has reached the level the where the application of these techniques to the screening of foods warrants further investigation.