PCR-restriction fragment length polymorphism analysis of a diagnostic 452-base-pair DNA fragment discriminates between Cryptosporidium parvum and C. meleagridis and between C. parvum isolates of human and animal origin

Appl Environ Microbiol. 2002 Apr;68(4):2071-6. doi: 10.1128/AEM.68.4.2071-2076.2002.

Abstract

Genomic DNAs from human Cryptosporidium isolates previously typed by analysis of the 18S ribosomal DNA locus (Cryptosporidium parvum bovine genotype, C. parvum human genotype, Cryptosporidium meleagridis, and Cryptosporidium felis) were used to amplify the diagnostic fragment described by Laxer et al. (M. A. Laxer, B. K. Timblin, and R. J. Patel, Am. J. Trop. Med. Hyg., 45:688-694, 1991). The obtained 452-bp amplified fragments were sequenced and aligned with the homologous Cryptosporidium wrairi sequence. Polymorphism was exploited to develop a restriction fragment length polymorphism method able to discriminate Cryptosporidium species and C. parvum genotypes.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Pairing / genetics*
  • Base Sequence
  • Cattle
  • Cattle Diseases / parasitology
  • Cryptosporidiosis / parasitology*
  • Cryptosporidiosis / veterinary
  • Cryptosporidium / classification*
  • Cryptosporidium / genetics
  • Cryptosporidium parvum / classification*
  • Cryptosporidium parvum / genetics
  • DNA, Protozoan* / genetics
  • DNA, Ribosomal / analysis
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • RNA, Ribosomal, 18S / genetics

Substances

  • DNA, Protozoan
  • DNA, Ribosomal
  • RNA, Ribosomal, 18S

Associated data

  • GENBANK/AF400130
  • GENBANK/AF400131
  • GENBANK/AF400132
  • GENBANK/AF400133