Abstract
Gaudichaudione A, a cytotoxic xanthone obtained from Garcinia gaudichaudii, displayed strong growth inhibitory effects against Jurkat human leukemic cells, parental murine leukemic P388 and P388/DOX-resistant cell lines, and exhibited similar cytotoxicity against P388/DOX as well as P388, but was less toxic toward normal human Chang liver cells. A variety of apoptotic events induced by gaudichaudione A at the mitochondrial and nuclear levels has been characterized. It appears that gaudichaudione A induced a collapse of the mitochondrial transmembrane potential. Caspase-3 was activated and induced internucleosomal DNA fragmentation and externalization of PS residues. The apoptotic features were also observed in ultrastructural studies.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antineoplastic Agents, Phytogenic / pharmacology
-
Apoptosis / drug effects*
-
Bridged-Ring Compounds / chemistry
-
Bridged-Ring Compounds / isolation & purification
-
Bridged-Ring Compounds / pharmacology*
-
Caspase 3
-
Caspases / drug effects
-
Caspases / metabolism*
-
DNA Fragmentation / drug effects
-
Dose-Response Relationship, Drug
-
Garcinia*
-
Humans
-
Jurkat Cells / drug effects
-
Jurkat Cells / ultrastructure
-
Membrane Potentials / drug effects
-
Mice
-
Microscopy, Electron
-
Mitochondria / drug effects*
-
Plant Extracts / chemistry
-
Plant Extracts / pharmacology
-
Tumor Cells, Cultured
-
Xanthenes / chemistry
-
Xanthenes / isolation & purification
-
Xanthenes / pharmacology*
-
Xanthones*
Substances
-
Antineoplastic Agents, Phytogenic
-
Bridged-Ring Compounds
-
Plant Extracts
-
Xanthenes
-
Xanthones
-
gaudichaudione A
-
xanthone
-
CASP3 protein, human
-
Casp3 protein, mouse
-
Caspase 3
-
Caspases