Selection of Gongronella butleri strains for enhanced chitosan yield with UV mutagenesis

J Biotechnol. 2002 May 9;95(2):189-93. doi: 10.1016/s0168-1656(02)00004-4.

Abstract

This paper describes the selection of Gongronella butleri strains producing higher chitosan yield using UV mutagenesis. We have devised an enzyme-linked immunosorbent assay for the selection of high chitin deacetylase (CDA) yielding strains. Mutant strains M+1, M+2 and M+7 could produce twice the extractable chitosan yield and double the CDA activity, as compared to the wild type strain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amidohydrolases / analysis
  • Amidohydrolases / immunology
  • Amidohydrolases / metabolism*
  • Antibodies
  • Antigens / analysis
  • Biotechnology
  • Chitin / analogs & derivatives
  • Chitin / biosynthesis*
  • Chitosan
  • Enzyme-Linked Immunosorbent Assay
  • Mucorales / enzymology*
  • Mucorales / genetics*
  • Mutagenesis
  • Ultraviolet Rays

Substances

  • Antibodies
  • Antigens
  • Chitin
  • Chitosan
  • Amidohydrolases
  • chitin deacetylase