Abstract
This paper describes the selection of Gongronella butleri strains producing higher chitosan yield using UV mutagenesis. We have devised an enzyme-linked immunosorbent assay for the selection of high chitin deacetylase (CDA) yielding strains. Mutant strains M+1, M+2 and M+7 could produce twice the extractable chitosan yield and double the CDA activity, as compared to the wild type strain.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amidohydrolases / analysis
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Amidohydrolases / immunology
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Amidohydrolases / metabolism*
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Antibodies
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Antigens / analysis
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Biotechnology
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Chitin / analogs & derivatives
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Chitin / biosynthesis*
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Chitosan
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Enzyme-Linked Immunosorbent Assay
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Mucorales / enzymology*
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Mucorales / genetics*
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Mutagenesis
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Ultraviolet Rays
Substances
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Antibodies
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Antigens
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Chitin
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Chitosan
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Amidohydrolases
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chitin deacetylase