Abstract
The immunological activities of five synthetic peptides of the prM protein of dengue-2 (DEN-2) virus containing B cell epitopes were evaluated in BALB/c mice. Two peptides elicited neutralizing antibodies against all four DEN serotypes. Virus-specific proliferative responses were demonstrated in mice immunized with four of the five peptides, demonstrating the presence of T cell epitopes. Mice immunized with three of the five peptides conjugated with bovine albumin showed statistically significant levels (P<0.05) of protection when challenged with DEN-2 virus. These results could constitute the basis for the establishment of the role of DEN virus pre and M antigens in the development of anti-flaviviral vaccines.
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Viral / biosynthesis
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Antigens, Viral / genetics
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B-Lymphocytes / immunology
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Dengue / immunology
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Dengue / prevention & control
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Dengue Virus / genetics
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Dengue Virus / immunology*
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Epitopes / genetics
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Epitopes / immunology
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In Vitro Techniques
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Lymphocyte Activation
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Male
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Neutralization Tests
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Sequence Homology, Amino Acid
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T-Lymphocytes / immunology
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Vaccines, Synthetic / genetics
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Vaccines, Synthetic / pharmacology
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Viral Proteins / chemical synthesis
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Viral Proteins / genetics
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Viral Proteins / immunology*
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Viral Vaccines / genetics
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Viral Vaccines / pharmacology
Substances
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Antibodies, Viral
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Antigens, Viral
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Epitopes
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Vaccines, Synthetic
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Viral Proteins
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Viral Vaccines