Abstract
Surrogate light chain expression during B lineage differentiation was examined by using indicator fluorochrome-filled liposomes in an enhanced immunofluorescence assay. Pro-B cells bearing surrogate light chain components were found in mice, but not in humans. A limited subpopulation of relatively large pre-B cells in both species expressed pre-B cell receptors. These cells had reduced expression of the recombinase activating genes, RAG-1 and RAG-2. Their receptor-negative pre-B cell progeny were relatively small, expressed RAG-1 and RAG-2, and exhibited selective down-regulation of VpreB and lambda5 expression. Comparative analysis of the 2 pre-B cell subpopulations indicated that loss of the pre-B cell receptors from surrogate light chain gene silencing was linked with exit from the cell cycle and light chain gene rearrangement to achieve B-cell differentiation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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B-Lymphocytes / cytology
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B-Lymphocytes / immunology*
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Bone Marrow Cells / cytology
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Bone Marrow Cells / immunology
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Cell Differentiation
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Cell Line
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DNA Primers
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DNA-Binding Proteins / genetics
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Gene Expression Regulation / immunology
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Gene Silencing
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Genes, RAG-1 / genetics
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Humans
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Immunoglobulin Light Chains / genetics*
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Immunoglobulin Light Chains, Surrogate
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Immunoglobulin lambda-Chains / genetics
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Membrane Glycoproteins / genetics
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Mice
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Nuclear Proteins
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Receptors, Antigen, B-Cell / analysis
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Reverse Transcriptase Polymerase Chain Reaction
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Species Specificity
Substances
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DNA Primers
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DNA-Binding Proteins
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Immunoglobulin Light Chains
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Immunoglobulin Light Chains, Surrogate
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Immunoglobulin lambda-Chains
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Membrane Glycoproteins
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Nuclear Proteins
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RAG2 protein, human
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Rag2 protein, mouse
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Receptors, Antigen, B-Cell
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V(D)J recombination activating protein 2