Abstract
Beta-mannosidase is an exoglycosidase involved in the degradation of N-linked oligosaccharides moieties of glycoproteins. Lack of beta-mannosidase activity leads to the lysosomal disorder beta-mannosidosis (MIM 248510). We have isolated and sequenced the gene encoding the mouse beta-mannosidase. Comparison of the deduced amino acid sequence of mouse, human, bovine, and goat beta-mannosidase showed 64%, identity, reflecting a high degree of evolutionary conservation. Analysis of a multiple tissue northern blotting revealed a major transcript of about 3.7 kb in all tissues examined. The northern analysis also demonstrates that there is differential tissue mRNA expression. The mouse beta-mannosidase gene (Bmn) was mapped to the distal end of Chromosome (Chr) 3, in a region that is homologous with a segment of human Chr 4 containing the orthologous human gene.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cattle
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Cell Nucleus / enzymology*
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Cell Nucleus / genetics
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Chromosome Mapping
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Chromosomes / enzymology*
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Chromosomes / genetics
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Chromosomes, Human, Pair 4 / genetics
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Cloning, Molecular
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DNA, Complementary / analysis
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DNA, Complementary / genetics
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Eukaryotic Cells / enzymology*
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Evolution, Molecular
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Gene Expression Regulation, Enzymologic / physiology
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Goats / genetics
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Humans
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Lysosomal Storage Diseases / enzymology*
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Lysosomal Storage Diseases / genetics
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Lysosomal Storage Diseases / physiopathology
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Lysosomes / enzymology*
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Mannosidases / genetics*
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Mannosidases / isolation & purification*
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Mice
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Molecular Sequence Data
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Phylogeny
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RNA, Messenger / metabolism
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Sequence Homology, Amino Acid
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Sequence Homology, Nucleic Acid
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Viscera / enzymology
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beta-Mannosidase
Substances
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DNA, Complementary
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RNA, Messenger
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Mannosidases
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beta-Mannosidase