Electrical contacting of glucose oxidase by surface-reconstitution of the apo-protein on a relay-boronic acid-FAD cofactor monolayer

Maya Zayats; Eugenii Katz; Itamar Willner

doi:10.1021/ja025503e

Electrical contacting of glucose oxidase by surface-reconstitution of the apo-protein on a relay-boronic acid-FAD cofactor monolayer

J Am Chem Soc. 2002 Mar 13;124(10):2120-1. doi: 10.1021/ja025503e.

Authors

Maya Zayats¹, Eugenii Katz, Itamar Willner

Affiliation

¹ Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.

PMID: 11878958
DOI: 10.1021/ja025503e

Abstract

A new methodology for the surface-reconstitution of apo-flavoenzymes on a relay unit (pyrroloquinoline quinone, PQQ) functionalized with a boronic acid ligand as a linker to native FAD was developed. The reconstitution of apo-glucose oxidase (apo-GOx) on the PQQ-FAD monolayer yields an electrically contacted enzyme-electrode.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Boronic Acids / chemistry*
Electrochemistry
Flavin-Adenine Dinucleotide / chemistry*
Glucose Oxidase / chemistry*
Oxidation-Reduction

Substances

Boronic Acids
Flavin-Adenine Dinucleotide
Glucose Oxidase