Heat shock proteome of Agrobacterium tumefaciens: evidence for new control systems

J Bacteriol. 2002 Mar;184(6):1772-8. doi: 10.1128/JB.184.6.1772-1778.2002.

Abstract

The regulation of Agrobacterium tumefaciens heat shock genes involves a transcriptional activator (RpoH) and repressor elements (HrcA-CIRCE). Using proteome analysis and mutants in these control elements, we show that the heat shock induction of 32 (out of 56) heat shock proteins is independent of RpoH and HrcA. These results indicate the existence of additional regulatory factors in the A. tumefaciens heat shock response.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agrobacterium tumefaciens / metabolism
  • Agrobacterium tumefaciens / physiology*
  • Autoradiography
  • Bacterial Proteins / genetics
  • DNA-Binding Proteins
  • Electrophoresis, Gel, Two-Dimensional
  • Gene Expression Regulation, Bacterial
  • Heat-Shock Proteins / genetics
  • Heat-Shock Response / physiology*
  • Hot Temperature
  • Mutation
  • Proteome / analysis
  • Proteome / metabolism*
  • Repressor Proteins / genetics
  • Sigma Factor*
  • Transcription Factors / genetics

Substances

  • Bacterial Proteins
  • DNA-Binding Proteins
  • Heat-Shock Proteins
  • Proteome
  • Repressor Proteins
  • Sigma Factor
  • Transcription Factors
  • heat-shock sigma factor 32