Generation of minimal protein identifiers of proteins from two-dimensional gels and recombinant proteins

Electrophoresis. 2002 Feb;23(4):621-5. doi: 10.1002/1522-2683(200202)23:4<621::AID-ELPS621>3.0.CO;2-J.

Abstract

We describe the technical feasibility and methodology to characterize a protein by a minimal set of structural information generated by matrix assisted laser desorption/ionization (MALDI)-mass spectrometry, termed a "minimal protein Identifier" (MPI). MPIs can be determined for proteins from two-dimensional gels and recombinant proteins and can be used to compare and identify proteins from these sources.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brain / embryology
  • Cloning, Molecular
  • Databases, Protein
  • Electrophoresis, Gel, Two-Dimensional / methods*
  • Fetus
  • Humans
  • Peptide Fragments / analysis
  • Peptide Library
  • Proteins / analysis*
  • Proteome / analysis
  • Recombinant Proteins / analysis
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

  • Peptide Fragments
  • Peptide Library
  • Proteins
  • Proteome
  • Recombinant Proteins