O-Specific chain structure from the lipopolysaccharide fraction of Pseudomonas reactans: a pathogen of the cultivated mushrooms

Carbohydr Res. 2002 Mar 1;337(5):467-71. doi: 10.1016/s0008-6215(02)00004-6.

Abstract

An O-specific polysaccharide containing 2-acetamidino-2-deoxy-beta-D-glucopyranose (Glcp2Am), 2,4-diacetamido-2,4,6-trideoxy-beta-D-glucopyranose (QuipNAc4NAc, bacillosamine) and 2,4-di-(N-acetyl-L-alanylamino)-2,4,6-trideoxy-beta-D-glucopyranose (QuipNAlaAc4NAlaAc) was isolated from the phenol-soluble lipopolysaccharide fraction of the mushroom-associated bacterium Pseudomonas reactans. The structure, determined by means of chemical analysis and 1D and 2D NMR spectroscopy, showed a linear trisaccharide-repeating unit, as shown below:-->3)-beta-D-QuipNAlaAc4NAlaAc-(1-->3)-alpha-D-Glcp2Am-(1-->3)-alpha-D-QuipNAc4NAc(1-->To our knowledge, this is the first complete O-chain structure reported for the lipopolysaccharide of a mushroom-associated bacterium.

MeSH terms

  • Agaricales
  • Carbohydrate Sequence
  • Lipopolysaccharides / chemistry*
  • Lipopolysaccharides / isolation & purification
  • O Antigens / chemistry*
  • O Antigens / isolation & purification
  • Pseudomonas / chemistry*
  • Trisaccharides / chemistry

Substances

  • Lipopolysaccharides
  • O Antigens
  • Trisaccharides