Rapid diagnosis of enterovirus infections by real-time PCR on the LightCycler using the TaqMan format

Diagn Microbiol Infect Dis. 2002 Feb;42(2):99-105. doi: 10.1016/s0732-8893(01)00330-3.

Abstract

A 2 h single-tube, reverse-transcription(RT)-PCR/hybridization assay using the TaqMan format for rapid diagnostic screening of enterovirus (EV) infections was optimized for the real-time LightCycler (LC) technology. For low EV load clinical samples an additional 30 min reamplification step using a novel primer-mix/probe design resulted in a 100% concordance with AMPLICOR EV PCR Test and in-house RT-PCR. Combined with maximum specificity, the sensitivity of LC-PCR was 10- to 100-fold higher compared to AMPLICOR EV Test.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Enterovirus / genetics
  • Enterovirus / isolation & purification*
  • Enterovirus Infections / diagnosis*
  • Enterovirus Infections / virology
  • Feces / virology
  • Fluorescent Dyes*
  • Humans
  • Pharynx / virology
  • Polymerase Chain Reaction / methods*
  • RNA, Viral / analysis
  • RNA, Viral / blood
  • RNA, Viral / cerebrospinal fluid
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sensitivity and Specificity
  • Taq Polymerase / metabolism*
  • Time Factors

Substances

  • Fluorescent Dyes
  • RNA, Viral
  • Taq Polymerase