Abstract
Fractionation of a methanol extract of the leaves and twigs of Casearia sylvestris, as directed by activity against KB cell cytotoxicity, led to the isolation of three novel clerodane diterpenoids, casearvestrins A-C (1-3). The structures of 1-3 were deduced from one- and two-dimensional NMR experiments, including relative stereochemical assignments based on ROESY correlations and COSY coupling constants. All three compounds displayed promising bioactivity, both in cytotoxicity assays against a panel of tumor cell lines and in antifungal assays via the growth inhibition of Aspergillus niger in a disk diffusion assay.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antifungal Agents / chemistry
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Antifungal Agents / isolation & purification*
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Antifungal Agents / pharmacology
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Antineoplastic Agents, Phytogenic / chemistry
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Antineoplastic Agents, Phytogenic / isolation & purification*
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Antineoplastic Agents, Phytogenic / pharmacology
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Aspergillus niger / drug effects
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Colonic Neoplasms
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Diterpenes / chemistry
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Diterpenes / isolation & purification*
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Diterpenes / pharmacology
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Drug Screening Assays, Antitumor
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Ecuador
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Female
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Humans
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Inhibitory Concentration 50
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KB Cells / drug effects
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Lung Neoplasms
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Magnoliopsida / chemistry*
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Molecular Conformation
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Molecular Structure
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Nuclear Magnetic Resonance, Biomolecular
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Ovarian Neoplasms
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Plant Leaves / chemistry
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Plant Shoots / chemistry
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Plants, Medicinal / chemistry*
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Spectrophotometry, Infrared
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Spectrophotometry, Ultraviolet
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Spectroscopy, Fourier Transform Infrared
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Tumor Cells, Cultured / drug effects
Substances
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Antifungal Agents
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Antineoplastic Agents, Phytogenic
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Diterpenes
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casearvestrin A
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casearvestrin B
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casearvestrin C