Abstract
Photosensitization of HEC1-B cells with a low concentration of hypericin and doses of light below 10 J/cm(2) caused cell death (apoptosis occurred mainly at doses between 2 and 5 J/cm(2), whereas necrosis prevailed above 6 J/cm(2)). However, pre-exposure of cells to innocuous irradiation (2 J/cm(2)) and successive challenge with a light dose that normally induced apoptosis (5 J/cm(2)) altered the expression of the proteins involved in the regulation of apoptosis, stress response and cell cycle. This change resulted in a significant increase in cell photo-tolerance.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Anthracenes
-
Apoptosis
-
Biological Transport
-
Carcinoma / drug therapy*
-
Carcinoma / genetics
-
Carcinoma / pathology
-
Endometrial Neoplasms / drug therapy*
-
Endometrial Neoplasms / genetics
-
Endometrial Neoplasms / pathology
-
Female
-
Gene Expression Regulation, Neoplastic
-
HSP70 Heat-Shock Proteins / genetics
-
Humans
-
Necrosis
-
Perylene / analogs & derivatives*
-
Perylene / metabolism
-
Perylene / pharmacology*
-
Photochemotherapy*
-
Proto-Oncogene Proteins / genetics
-
Proto-Oncogene Proteins c-bcl-2 / genetics
-
Radiation-Sensitizing Agents / metabolism
-
Radiation-Sensitizing Agents / pharmacology*
-
bcl-2-Associated X Protein
-
bcl-X Protein
Substances
-
Anthracenes
-
BCL2L1 protein, human
-
HSP70 Heat-Shock Proteins
-
Proto-Oncogene Proteins
-
Proto-Oncogene Proteins c-bcl-2
-
Radiation-Sensitizing Agents
-
bcl-2-Associated X Protein
-
bcl-X Protein
-
Perylene
-
hypericin