Abstract
Hypohalites (OCl-, OBr-) are formed at inflammation sites as antimicrobial agents. OCl- is also used for the disinfection of water supplies and the association of drinking chlorinated water with cancer risk is pointed out. In this study, OCl- itself induced 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) formation, while OBr- damaged DNA only when glutathione (GSH) was added. OCl- caused oxidative DNA damage more efficiently than OBr-/GSH. In experiment with 32P-labeled DNA fragments, OCl- strongly caused piperidine-labile sites at guanine residues than piperidine-inert 8-oxodG, whereas OBr-/GSH caused no piperidine-labile sites. Endogenous OCl- may play a role in genotoxicity close to the site of inflammation.
MeSH terms
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8-Hydroxy-2'-Deoxyguanosine
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Animals
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Bromates / pharmacology*
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Carcinogens / pharmacology*
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Cattle
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Cell Line
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DNA / drug effects*
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DNA Damage / drug effects*
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Deoxyguanosine / analogs & derivatives
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Deoxyguanosine / metabolism*
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Dimethyl Sulfoxide / metabolism
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Electron Spin Resonance Spectroscopy
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Genes, Tumor Suppressor
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Genes, p53 / physiology
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Genes, ras / physiology
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Glutathione / metabolism
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Humans
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Inflammation / etiology*
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Neoplasms / etiology*
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Oxidation-Reduction
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Phosphorus Isotopes
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Piperidines / metabolism
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Sodium Hypochlorite / pharmacology*
Substances
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Bromates
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Carcinogens
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Phosphorus Isotopes
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Piperidines
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piperidine
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8-Hydroxy-2'-Deoxyguanosine
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DNA
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Sodium Hypochlorite
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Deoxyguanosine
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Glutathione
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hypobromous acid
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Dimethyl Sulfoxide