Induction of apoptosis in HL-60 cells by photochemically generated hydroxyl radicals

Bioorg Med Chem Lett. 2002 Feb 25;12(4):675-6. doi: 10.1016/s0960-894x(01)00830-7.

Abstract

Reactive oxygen species (ROS), especially hydroxyl radicals are postulated to mediate apoptosis of the cell. Here we demonstrate that hydroxyl radicals generated selectively by photolysis of a photo-Fenton reagent, N,N'-bis(2-hydroperoxy-2-methoxyethyl)-1,4,5,8-naphthaldiimide (NP-III), induce apoptosis in HL-60 (human promyelocytic leukemia) cells involving the activation of caspase-3.

MeSH terms

  • Apoptosis / drug effects*
  • Caspase 3
  • Caspases / analysis
  • Caspases / metabolism
  • HL-60 Cells / drug effects*
  • Humans
  • Hydroxyl Radical / pharmacology*
  • Hydroxyl Radical / radiation effects
  • Naphthalenes
  • Photochemistry
  • Radiation-Sensitizing Agents
  • Reactive Oxygen Species / pharmacology
  • Reactive Oxygen Species / radiation effects
  • Ultraviolet Rays

Substances

  • Naphthalenes
  • Radiation-Sensitizing Agents
  • Reactive Oxygen Species
  • N,N'-bis(2-hydroxyperoxy-2-methoxyethyl)-1,4,5,8-naphthalenetetracarboxylic diimide
  • Hydroxyl Radical
  • CASP3 protein, human
  • Caspase 3
  • Caspases