[Molecular cloning of specific porcine adipocyte membrane protein cDNA]

Yi Chuan Xue Bao. 2002 Jan;29(1):7-11.
[Article in Chinese]

Abstract

Specific porcine adipocyte membrane protein (PAMP), which was recently reported, is probably associated with the growth and development of adipocyte and regulator of adipose tissue. Using RT-PCR and RACE methods, and primer designed according to published sequence of PAMP gene 5' end, we have successfully amplified about a 500 bp and a 400 bp from pig adipose tissue. A 500 bp fragment was retrieved, cloned and sequenced. The sequencing result showed that we have got the clone and sequence of cDNA 5'-end for PAMP gene.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / physiology*
  • Adipose Tissue / growth & development*
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary / analysis
  • Membrane Proteins / genetics*
  • Membrane Proteins / physiology
  • Molecular Sequence Data
  • Reverse Transcriptase Polymerase Chain Reaction
  • Swine

Substances

  • DNA, Complementary
  • Membrane Proteins