Chlorogentisylquinone, a new neutral sphingomyelinase inhibitor, produced by a marine fungus

J Antibiot (Tokyo). 2001 Nov;54(11):882-9. doi: 10.7164/antibiotics.54.882.

Abstract

Chlorogentisylquinone, a new inhibitor of neutral sphingomyelinase activity, was purified from the culture broth of a fungal strain FOM-8108 isolated from a marine environment by solvent extraction, silica gel chromatography and Sephadex LH-20 chromatography. Its chemical structure was elucidated by spectroscopic studies including 1H, 13C, DEPT, HMQC and HMBC NMR experiments. Chlorogentisylquinone inhibited neutral sphingomyelinase activity of rat brain membranes with an IC50 value of 1.2 microM.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, Gel
  • Chromatography, High Pressure Liquid
  • Enzyme Inhibitors / chemistry
  • Enzyme Inhibitors / isolation & purification*
  • Enzyme Inhibitors / pharmacology
  • Fermentation
  • Fungi / chemistry
  • Fungi / metabolism*
  • Kinetics
  • Magnetic Resonance Spectroscopy
  • Microbial Sensitivity Tests
  • Quinones / chemistry
  • Quinones / isolation & purification*
  • Sphingomyelin Phosphodiesterase / antagonists & inhibitors*
  • Tumor Cells, Cultured

Substances

  • Enzyme Inhibitors
  • Quinones
  • gentisylquinone
  • chlorogentisylquinone
  • Sphingomyelin Phosphodiesterase