Abstract
We have examined the efficiency of DNA photooxidation in DNA/PNA duplex and DNA/(PNA)(2) triplex for the first time. DNA/PNA duplex was cleaved at GG steps by external riboflavin with high efficiency like specific GG cleavage in DNA/DNA duplex. However, the 5'G selectivity of the GG oxidation in DNA/PNA duplex was much lower than that observed in DNA/DNA duplex. Remote DNA oxidation of oxidant-tethered DNA/PNA duplex was considerably suppressed. In contrast, the formation of DNA/(PNA)(2) triplex by hybridization with two PNA strands completely inhibited the remote GG oxidation, indicating that PNA acts as an inhibition for remote oxidative DNA damage.
MeSH terms
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DNA / metabolism*
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DNA / radiation effects
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DNA Damage / drug effects*
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DNA Damage / radiation effects
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Dinucleoside Phosphates / metabolism
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Dinucleoside Phosphates / radiation effects
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Models, Molecular
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Nucleic Acid Hybridization*
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Oligodeoxyribonucleotides / chemical synthesis
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Oligodeoxyribonucleotides / metabolism
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Oligodeoxyribonucleotides / radiation effects
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Oxidants, Photochemical / pharmacology
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Oxidation-Reduction / drug effects
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Peptide Nucleic Acids / chemical synthesis
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Peptide Nucleic Acids / metabolism
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Peptide Nucleic Acids / pharmacology*
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Photochemistry
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Riboflavin / pharmacology
Substances
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Dinucleoside Phosphates
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Oligodeoxyribonucleotides
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Oxidants, Photochemical
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Peptide Nucleic Acids
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diguanosine triphosphate
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DNA
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Riboflavin