Abstract
Plasmodium vivax Duffy Binding Protein (Pv-DBP) is essential during merozoite invasion of reticulocytes. Reticulocyte binding region identification is important for understanding Pv-DBP reticulocyte recognition. Fifty 20 mer non-overlapping peptides, spanning Pv-DBP sequences, were tested in erythrocyte and reticulocyte binding assays. Ten HARBPs, mainly located in region II (Kd 50-130 nM), were High Activity Reticulocyte Binding Peptides (HARBPs); one bound to erythrocytes. Reticulocyte trypsin-, chymotrypsin- or neuraminidase- treatment affects HARBP binding differently, suggesting that these peptides have different reticulocyte-binding-sites. Some peptides bound to a Coomasie non-stainable 40 Kda band. Some HARBPs were able to block recombinant PvRII binding (Pv-DBP region II) to Duffy positive reticulocytes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens, Protozoan*
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Binding Sites
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Binding, Competitive
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Carrier Proteins / chemistry*
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Carrier Proteins / metabolism
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Cross-Linking Reagents / pharmacology
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Dose-Response Relationship, Drug
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Erythrocytes / cytology
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Erythrocytes / metabolism*
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Female
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Humans
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Ligands
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Molecular Sequence Data
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Peptide Biosynthesis
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Peptides / chemistry*
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Plasmodium vivax / chemistry*
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Plasmodium vivax / metabolism
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Protein Binding
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Protozoan Proteins*
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Receptors, Cell Surface / chemistry*
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Receptors, Cell Surface / metabolism
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Recombinant Proteins / metabolism
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Reticulocytes / cytology
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Reticulocytes / metabolism*
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beta-Thalassemia / blood
Substances
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Antigens, Protozoan
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Carrier Proteins
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Cross-Linking Reagents
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Duffy antigen binding protein, Plasmodium
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Ligands
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Peptides
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Protozoan Proteins
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Receptors, Cell Surface
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Recombinant Proteins