Abstract
Antigen-independent adhesive interactions between T lymphocytes and antigen-presenting cells (APCs) are essential for scanning for specific antigens on the APC surface and for initiating the immune response. Here we show, through time-lapse imaging of live cells, that the intercellular adhesion molecule 3 (ICAM-3, also known as CD50) is clustered specifically at the region of the T lymphocyte surface that initiates contact with APCs. We describe the role of ICAM-3 in T cell-APC conjugate formation before antigen recognition, in early intracellular signaling and in cytoskeletal rearrangement. Our data indicate that ICAM-3 is important in the initial scanning of the APC surface by T cells and, therefore, in generating the immune response.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing*
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Antigen Presentation*
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Antigen-Presenting Cells / immunology*
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Antigen-Presenting Cells / ultrastructure
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Antigens, CD*
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Antigens, Differentiation*
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Carrier Proteins / isolation & purification
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Cell Adhesion
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Cell Adhesion Molecules / isolation & purification*
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Cell Compartmentation
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Cytoskeleton / ultrastructure
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DNA-Binding Proteins / metabolism
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Humans
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Immunologic Capping
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Intercellular Junctions / immunology*
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Intercellular Junctions / ultrastructure
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Membrane Glycoproteins / isolation & purification
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Membrane Proteins*
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NFATC Transcription Factors
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Nuclear Proteins*
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Phosphoproteins / isolation & purification
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Phosphorylation
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Receptors, Antigen, T-Cell / metabolism
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Signal Transduction
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T-Lymphocytes / immunology*
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T-Lymphocytes / ultrastructure
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Transcription Factors / metabolism
Substances
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Adaptor Proteins, Signal Transducing
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Antigens, CD
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Antigens, Differentiation
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Carrier Proteins
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Cell Adhesion Molecules
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DNA-Binding Proteins
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ICAM3 protein, human
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LAT protein, human
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Membrane Glycoproteins
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Membrane Proteins
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NFATC Transcription Factors
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Nuclear Proteins
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P-selectin ligand protein
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Phosphoproteins
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Receptors, Antigen, T-Cell
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Transcription Factors