RNase H mediated cleavage of RNA by cyclohexene nucleic acid (CeNA)

Nucleic Acids Res. 2001 Dec 15;29(24):4941-7. doi: 10.1093/nar/29.24.4941.

Abstract

Cyclohexene nucleic acid (CeNA) forms a duplex with RNA that is more stable than a DNA-RNA duplex (DeltaTm per modification: +2 degrees C). A cyclohexenyl A nucleotide adopts a 3'-endo conformation when introduced in dsDNA. The neighbouring deoxynucleotide adopts an O4'-endo conformation. The CeNA:RNA duplex is cleaved by RNase H. The Vmax and Km of the cleavage reaction for CeNA:RNA and DNA:RNA is in the same range, although the kcat value is about 600 times lower in the case of CeNA:RNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cyclohexanes / chemistry*
  • Cyclohexanes / metabolism
  • Cyclohexenes
  • Kinetics
  • Magnetic Resonance Spectroscopy / methods
  • Nucleic Acid Conformation
  • Oligonucleotides / chemistry
  • Oligonucleotides / metabolism
  • RNA / chemistry*
  • RNA / metabolism
  • Ribonuclease H / chemistry*
  • Ribonuclease H / metabolism
  • Temperature

Substances

  • Cyclohexanes
  • Cyclohexenes
  • Oligonucleotides
  • cyclohexene
  • RNA
  • Ribonuclease H