Objective: To screen related gene of endometrial cancer by mRNA differential display (DD)-polymerase chain reaction (PCR).
Methods: Individual mRNA from normal and neoplastic endometrium obtained from the same patient were comparatively studied by DD methodology. The mRNAs were isolated from the tissues, reverse transcribed to cDNA, and then amplified by PCR. The PCR products were displayed on a polyacrylamid gel by silver staining, and the differentially expressed bands were retrieved and reamplified. Subsequently we selected four bands for sequencing and making further investigation.
Results: cDNA homology was analysed by searching through GenBank sequence databases and indicated that tumor fragment (T1.7) has no significant sequence homology with any sequence of known function. Acession number is AF315581. Further study showed that T1.7 expressed more abundantly in endometrial cancer than hyperplasia endometrium, but not expressed in normal endometrium.
Conclusions: T1.7 is a new expressed sequence tag. It may be related gene fragment of endometrial cancer.