Abstract
In conclusion, by taking advantage of the overall sequence homology and structural similarity of G alpha subunits, functional chimeric G alpha subunits can be generated and used as tools for the identification of sequence-specific factors that mediate receptor: G protein specificity. The [35S]GTP gamma S binding assay and the affinity shift activity assay are two sensitive biochemical approaches that can be used to assess receptor: G protein coupling in vitro. These in vitro assays limit confounding influences from cellular proteins and allow for the strict control of receptor: G protein ratios.
MeSH terms
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Animals
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Cattle
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Cell Line
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Cell Membrane / metabolism
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GTP-Binding Protein alpha Subunits, Gq-G11
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Guanosine 5'-O-(3-Thiotriphosphate) / metabolism
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Heterotrimeric GTP-Binding Proteins / isolation & purification
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Heterotrimeric GTP-Binding Proteins / metabolism*
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Kinetics
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Protein Binding
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Protein Subunits
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Radioisotope Dilution Technique
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Receptors, Cell Surface / metabolism*
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Recombinant Fusion Proteins / metabolism
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Recombinant Proteins / metabolism
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Rod Cell Outer Segment / metabolism
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Sensitivity and Specificity
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Spodoptera
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Sulfur Radioisotopes
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Transfection
Substances
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Protein Subunits
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Receptors, Cell Surface
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Recombinant Fusion Proteins
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Recombinant Proteins
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Sulfur Radioisotopes
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Guanosine 5'-O-(3-Thiotriphosphate)
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GTP-Binding Protein alpha Subunits, Gq-G11
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Heterotrimeric GTP-Binding Proteins