Abstract
Gene vmrA, cloned from Vibrio parahaemolyticus, made Escherichia coli resistant to 4',6-diamino-2-phenylindol, tetraphenylphosphonium chloride, acriflavine, and ethidium bromide. VmrA belongs to the DinF branch of MATE family efflux transporters. VmrA catalyzed acriflavine efflux and showed Na(+)/drug transporter activity because the addition of tetraphenylphosphonium to Na(+)-loaded cells caused Na(+) efflux.
MeSH terms
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Acriflavine / metabolism
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Anti-Infective Agents, Local / metabolism
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Antiporters / classification
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Antiporters / genetics*
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Antiporters / metabolism
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Bacterial Proteins / classification
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Base Sequence
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Cloning, Molecular / methods
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DNA, Bacterial
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Escherichia coli / drug effects
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Escherichia coli / metabolism
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Fluorescent Dyes / metabolism
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Genes, Bacterial
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Molecular Sequence Data
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Sequence Analysis, DNA
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Sodium-Potassium-Exchanging ATPase / classification
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Sodium-Potassium-Exchanging ATPase / genetics*
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Sodium-Potassium-Exchanging ATPase / metabolism
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Vibrio parahaemolyticus / drug effects
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Vibrio parahaemolyticus / genetics*
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Vibrio parahaemolyticus / metabolism
Substances
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Anti-Infective Agents, Local
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Antiporters
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Bacterial Proteins
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DNA, Bacterial
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Fluorescent Dyes
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VmrA protein, Vibrio parahaemolyticus
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Acriflavine
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Sodium-Potassium-Exchanging ATPase