Effects of erysimin G on renal tubular function and 70-pS K+ channel activity of thick ascending limb

Acta Pharmacol Sin. 2001 May;22(5):411-4.

Abstract

Aim: To study the effect of erysimin G (CH35H52O13) on the thick ascending limb (TAL) 70-pS K+ channel of rat kidney and its effect on diuresis.

Methods: The patch-clamp cell-attached recording technique was used to record the single potassium channel current, and the urine volume (UV) was measured by urethral intubation to determine the diuretic effect.

Results: Erysimin G can increase the urine volume and decrease the 70-pS potassium channel activity of TAL.

Conclusion: Erysimin G has a diuretic effect and its inhibition on the activity of apical 70-pS potassium channel may be the mechanism of its diuretic effect.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cardiac Glycosides / isolation & purification
  • Cardiac Glycosides / pharmacology*
  • Diuretics / isolation & purification
  • Diuretics / pharmacology*
  • Erysimum / chemistry
  • Female
  • Kidney Tubules, Distal / physiology*
  • Male
  • Patch-Clamp Techniques
  • Plants, Medicinal / chemistry
  • Potassium Channels / drug effects
  • Potassium Channels / metabolism*
  • Rabbits
  • Random Allocation
  • Rats
  • Urine

Substances

  • Cardiac Glycosides
  • Diuretics
  • Potassium Channels
  • erysimin G