A phospholipase C inhibitor, phenylmethylsulfonyl fluoride, ameliorates ischemic injury to brain mitochondria in rats

Acta Pharmacol Sin. 2001 Mar;22(3):249-52.

Abstract

Aim: To study the effect of phenylmethylsulfonyl fluoride (PMSF), a phospholipase C inhibitor, on ischemic injury to brain mitochondria in rats.

Methods: The phospholipid level, membrane fluidity, and respiratory control ratio of brain mitochondria were measured. The effect of phenylmethylsulfonyl fluoride was tested.

Results: The phospholipid level, membrane fluidity, and respiratory control ratio of brain mitochondria in ischemia group decreased but increased in the PMSF treatment group (P < 0.05).

Conclusion: PMSF ameliorated ischemic injury to brain mitochondria in rats.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / metabolism*
  • Brain Ischemia / metabolism*
  • Male
  • Membrane Fluidity / drug effects
  • Mitochondria / metabolism*
  • Mitochondria / pathology
  • Phenylmethylsulfonyl Fluoride / pharmacology*
  • Phospholipids / metabolism
  • Rats
  • Rats, Wistar
  • Type C Phospholipases / antagonists & inhibitors*

Substances

  • Phospholipids
  • Phenylmethylsulfonyl Fluoride
  • Type C Phospholipases