Abstract
Two approaches for presentation of a part of the rickettsial outer membrane protein A (OmpA) of Rickettsia rickettsii, namely (1) recombinant Mycobacterium vaccae (rMV) or (2) recombinant DNA vaccine, stimulated protective immunity against a lethal challenge with the closely related bacterium, R. conorii, in mice. After primary immunization with rMV and booster immunization with homologous recombinant protein, 67 and 55% of mice were protected against challenge in two experiments. DNA vaccination with booster recombinant protein immunization protected six out of eight animals from a lethal challenge. Production of IFN-gamma by antigen-exposed T-lymphocytes of DNA vaccine recipients indicated that cellular immunity had been stimulated.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Bacterial Outer Membrane Proteins / genetics
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Bacterial Outer Membrane Proteins / immunology*
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Base Sequence
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DNA, Bacterial / genetics
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Escherichia coli / genetics
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Immunization, Secondary
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Interferon-gamma / biosynthesis
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Male
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Mice
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Mice, Inbred C3H
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Mycobacterium / genetics
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / immunology
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Rickettsia rickettsii / genetics
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Rickettsia rickettsii / immunology*
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Rickettsial Vaccines / genetics
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Rickettsial Vaccines / pharmacology*
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Rocky Mountain Spotted Fever / immunology
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Rocky Mountain Spotted Fever / prevention & control*
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T-Lymphocytes / immunology
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Transformation, Genetic
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Vaccines, DNA / genetics
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Vaccines, DNA / pharmacology
Substances
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Bacterial Outer Membrane Proteins
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DNA, Bacterial
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Recombinant Fusion Proteins
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Rickettsial Vaccines
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Vaccines, DNA
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Interferon-gamma