Abstract
In our previous studies, TPA treatment of LA-N-1 cells stimulated the production of diacylglycerol in nuclei, probably through the activation of a phospholipase C. Stimulation of the synthesis of nuclear phosphatidylcholine by the activation of CTP:phosphocholine cytidylyltransferase was also observed. The present data show that both effects were inhibited by the pretreatment of the cells with D609, a selective phosphatidylcholine-phospholipase C inhibitor, indicating that the diacylglycerol produced through the hydrolysis of phosphatidylcholine in the nuclei is reutilized for the synthesis of nuclear phosphatidylcholine and is required for the activation of CTP:phosphocholine cytidylyltransferase.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antioxidants / pharmacology*
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Bridged-Ring Compounds / pharmacology*
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Cell Division
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Cell Nucleus / metabolism*
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Choline-Phosphate Cytidylyltransferase / metabolism
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Diglycerides / metabolism
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Diglycerides / physiology*
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Enzyme Activation
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Enzyme Inhibitors / pharmacology
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Humans
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Hydrolysis
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Lipid Metabolism
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Neuroblastoma / metabolism*
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Norbornanes
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Phosphatidylcholines / metabolism*
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Phosphodiesterase Inhibitors / pharmacology*
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Thiocarbamates
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Thiones / pharmacology*
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Time Factors
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Tumor Cells, Cultured
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Type C Phospholipases / metabolism
Substances
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Antioxidants
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Bridged-Ring Compounds
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Diglycerides
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Enzyme Inhibitors
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Norbornanes
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Phosphatidylcholines
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Phosphodiesterase Inhibitors
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Thiocarbamates
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Thiones
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tricyclodecane-9-yl-xanthogenate
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Choline-Phosphate Cytidylyltransferase
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Type C Phospholipases