Measurement of Ca(2+) currents in intact slow skeletal muscle fibers of the frog by the three-microelectrode technique

Brain Res Brain Res Protoc. 2001 Dec;8(3):208-11. doi: 10.1016/s1385-299x(01)00118-0.

Abstract

The recording of currents passing through calcium channels in intact skeletal muscle fibers presents several difficulties. However, use of the three-microelectrode voltage-clamp technique at the end of the fiber provided us with a good approximation of current values in such fibers. Using this technique, we were able to measure the calcium-channel current in slow skeletal muscle fibers of the frog (Rana pipiens) and to quantify the effects of denervation on this current.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Barium
  • Calcium Channels / metabolism*
  • Electric Stimulation
  • In Vitro Techniques
  • Microelectrodes
  • Muscle Fibers, Slow-Twitch / metabolism*
  • Muscle Fibers, Slow-Twitch / physiology
  • Muscle, Skeletal / cytology
  • Muscle, Skeletal / metabolism*
  • Muscle, Skeletal / physiology
  • Patch-Clamp Techniques
  • Rana pipiens
  • Synaptic Transmission / physiology

Substances

  • Calcium Channels
  • Barium