Tumour-related enzyme alterations in the clear cell type of human renal cell carcinoma identified by two-dimensional gel electrophoresis

S Balabanov; U Zimmermann; C Protzel; C Scharf; K J Klebingat; R Walther

doi:10.1046/j.0014-2956.2001.02546.x

Tumour-related enzyme alterations in the clear cell type of human renal cell carcinoma identified by two-dimensional gel electrophoresis

Eur J Biochem. 2001 Nov;268(22):5977-80. doi: 10.1046/j.0014-2956.2001.02546.x.

Authors

S Balabanov¹, U Zimmermann, C Protzel, C Scharf, K J Klebingat, R Walther

Affiliation

¹ Department of Medical Biochemistry and Molecularbiology, University of Greifswald, Germany.

PMID: 11722587
DOI: 10.1046/j.0014-2956.2001.02546.x

Abstract

To identify tumour-related enzyme alterations we have used 2D-gels to analyse the proteome from dissected malignant and benign kidney areas from patients with clear-cell-type renal carcinoma. The expression of 12 proteins was diminished in tumour. Four proteins were characterized by mass spectrometry and were identified as enoyl-CoA hydratase, alpha-glycerol-3-phosphate dehydrogenase, aldehyde dehydrogenase 1 and aminoacylase-I.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aldehyde Dehydrogenase / metabolism*
Amidohydrolases / metabolism*
Base Sequence
Carcinoma, Renal Cell / enzymology*
Carcinoma, Renal Cell / pathology
DNA Primers
Electrophoresis, Gel, Two-Dimensional
Enoyl-CoA Hydratase / metabolism*
Glycerolphosphate Dehydrogenase / metabolism*
Humans
Kidney Neoplasms / enzymology*
Kidney Neoplasms / pathology
Peptide Mapping

Substances

DNA Primers
Glycerolphosphate Dehydrogenase
Aldehyde Dehydrogenase
Amidohydrolases
aminoacylase I
Enoyl-CoA Hydratase