Novel spermine-amino acid conjugates and basic tripeptides enhance cleavage of the hairpin ribozyme at low magnesium ion concentration

Bioorg Med Chem Lett. 2001 Dec 3;11(23):3007-10. doi: 10.1016/s0960-894x(01)00608-4.

Abstract

Combinations of the polyamine spermine and magnesium ions synergize to dramatically enhance cleavage of the hairpin ribozyme. Certain synthetic basic tripeptides stimulate hairpin cleavage significantly at limiting magnesium ion concentration, notably the tripeptide of L-diaminobutyric acid (Dab). Of a range of novel synthetic spermine-amino acid conjugates, L-Dab-spermine (but not D-Dab nor other amino acid conjugates) was more effective than spermine itself.

MeSH terms

  • Amino Acids / chemistry*
  • Amino Acids / metabolism
  • Aminobutyrates / chemistry
  • Biochemistry / methods
  • Magnesium / chemistry*
  • Nucleic Acid Conformation
  • Peptides / chemistry*
  • Peptides / metabolism
  • RNA, Catalytic / chemistry*
  • RNA, Catalytic / metabolism
  • Spermine / chemistry*
  • Spermine / metabolism

Substances

  • Amino Acids
  • Aminobutyrates
  • Peptides
  • RNA, Catalytic
  • Spermine
  • 2,4-diaminobutyric acid
  • Magnesium