It has been shown that ionophore of bivalent cations (IBC) isolated from fatless, subjected to partial triptic hydrolysis cattle heart or liver mitochondria decreases BPM resistance inducing Ca2+ conductivity. Ions of lanthane in micromolar concentrations decrease calcium conductivity induced with IBC. When ten-fold gradient in Ca2+ was created on BPM the intitiation of the membrane potential fo 9-11 mV was observed. The role fo IBC and water soluble factors binding Ca2+ with high affinity, in the mitochrondial mechanism of Ca2+ translocation is discussed.