[MALDI-mass spectrometry for identification of new proteins in snake venoms]

V V Kukhtina; K Weise; A V Osipov; V G Starkov; M I Titov; S E Esipov; T V Ovchinnikova; V I Tsetlin; Iu N Utkin

[MALDI-mass spectrometry for identification of new proteins in snake venoms]

Bioorg Khim. 2000 Nov;26(11):803-7.

[Article in Russian]

Authors

V V Kukhtina¹, K Weise, A V Osipov, V G Starkov, M I Titov, S E Esipov, T V Ovchinnikova, V I Tsetlin, Iu N Utkin

Affiliation

¹ Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP-7 Moscow, 117871 Russia.

PMID: 11696890

Abstract

By MALDI MS, we searched cobra venoms for new low-content polypeptides. A number of new proteins with molecular masses 7-25 kDa, characteristic of the known snake protein toxins, were identified, with the content of one of them less than 0.02%.

MeSH terms

Animals
Chromatography, Gel
Elapid Venoms / chemistry*
Elapid Venoms / isolation & purification
Molecular Weight
Peptides / chemistry*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Spectrophotometry, Ultraviolet
Toxins, Biological / chemistry*

Substances

Elapid Venoms
Peptides
Toxins, Biological