Hibiscus acid as an inhibitor of starch digestion in the Caco-2 cell model system

Biosci Biotechnol Biochem. 2001 Sep;65(9):2087-9. doi: 10.1271/bbb.65.2087.

Abstract

Hibiscus acid, an alpha-amylase inhibitor isolated from roselle tea, and its derivatives were compared in an inhibition test for starch digestion. An alpha-amylase-added Caco-2 system was established as a useful model to evaluate the effects of alpha-glucosidase inhibitors on starch digestion. Hibiscus acid showed weak inhibition in this model system, and the methyl ester derivatives showed even weaker or no acitivity.

MeSH terms

  • Animals
  • Caco-2 Cells
  • Citrates / pharmacology*
  • Citric Acid / pharmacology
  • Digestion / drug effects
  • Glucose / metabolism
  • Glycoside Hydrolase Inhibitors
  • Humans
  • Inhibitory Concentration 50
  • Pancreas / enzymology
  • Starch / metabolism*
  • Swine
  • alpha-Amylases / antagonists & inhibitors
  • alpha-Amylases / metabolism
  • alpha-Glucosidases / metabolism

Substances

  • Citrates
  • Glycoside Hydrolase Inhibitors
  • Citric Acid
  • hydroxycitric acid
  • Starch
  • alpha-Amylases
  • alpha-Glucosidases
  • Glucose