High-sensitivity negative-ion laser spray for liquid chromatography/mass spectrometry

Rapid Commun Mass Spectrom. 2001;15(21):2020-6. doi: 10.1002/rcm.473.

Abstract

A negative-ion mode laser spray interface for use in liquid chromatography/mass spectrometry (LC/MS) has been developed and investigated. The laser spray gave sensitivities that were orders of magnitude better than the negative-ion electrospray for all samples investigated with the exception of sugars. Fragment ions, HSO(-4)and SO(-3), were formed from the laser-sprayed aqueous solution of cholesterol 3-sulfate sodium salt. This suggests that structural information may be obtained directly from the laser-spray mass spectra for thermally labile compounds.

MeSH terms

  • Carbohydrate Sequence
  • Carbohydrates / analysis
  • Cholesterol / chemistry
  • Chromatography, Liquid
  • Cyclodextrins / chemistry
  • Lasers
  • Molecular Sequence Data
  • Spectrometry, Mass, Electrospray Ionization / methods*
  • Sulfates / chemistry

Substances

  • Carbohydrates
  • Cyclodextrins
  • Sulfates
  • Cholesterol