[Identification of Amomum villosum, Amomum villosum var. xanthioides and Amomum longiligulare on ITS-1 sequence]

H Pan; F Huang; P Wang; L Zhou; L Cao; R Liang

[Identification of Amomum villosum, Amomum villosum var. xanthioides and Amomum longiligulare on ITS-1 sequence]

Zhong Yao Cai. 2001 Jul;24(7):481-3.

[Article in Chinese]

Authors

H Pan¹, F Huang, P Wang, L Zhou, L Cao, R Liang

Affiliation

¹ Guangzhou University of TCM, Guangzhou 510405.

PMID: 11668737

Abstract

Objective: To identify Amomum villosum Lour. and some their adulterants on molecular biology.

Method: The DNA of Amomum villosum Lour. and some their adulterants were extracted, and amplified using ITS-1 primer. The amplificed DNA were purified and then sequenced by direct PCR sequencing method.

Result: The ITS-sequence of all of the samples are 248 bp in size. But there are 7 bases in Amomum villosum Lour var. xanthioides (Wall.ex Bak) T.L. Wu et Senjen and 12 hases in Amomum longiligulare T.L. Wu. differing from Amomum villosum Lour.

Conclusion: The ITS-1 sequence can be used to identify effectively Amomum villosum Lour. and their adulterants.

Publication types

English Abstract

MeSH terms

Amomum / genetics*
Base Sequence
Fruit / genetics
Molecular Sequence Data
Plants, Medicinal / genetics*
Polymerase Chain Reaction
Sequence Alignment
Sequence Analysis, DNA